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KMID : 0545120020120020296
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 2 p.296 ~ p.300
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Involvement of Lipopolysaccharide of Bradyrhizobium japonicum in Metal Binding
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OH, EUN TAEX
YUN, HYUN SHIK/HEO, TAE-RYEON/KOH, SUNG-CHEOL/OH, KYE-HEON/SO, JAE-SEONG
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Abstract
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Bacterial cell surface components are the major factors responsible for pathogenesis and bioremediation. In particular, the surface of a Gram-negative bacterium cell has a variety of components compared to that of a Gram-positive cell. In our previous study, we isolated an isogenic mutant of Bradyrhizobium japonicum, which exhibited altered cell surface characteristics, including an increased hydrophobicity. Polyacrylamide gel electrophoretic analysis of the lipopolysaccharide (LPS) in the mutant demonstrated that the O-polysaccharide part was completely absent. Meanwhile, a gel permeation chromatographic analysis of the exopolysaccharide (EPS) in the mutant demonstrated that it was unaltered. Since LPSs are known to have several anion groups that interact with various cation groups and metal ions, the mutant provided an opportunity to examine the direct role of LPS in metal binding by B. japonicum. Using atomic absorption spectrophotometry, it was clearly demonstrated that LPS was involved in metal binding. The binding capacity of the LPS mutant to various metal ions (Cd^2+, Cu^2+, Pb^2+, and Zn^2+) was 50-70% lower than that of the wild-type strain. Also, through an EPS analysis and desorption experiment, it was found that EPS and centrifugal force had no effect on the metal binding. Accordingly, it would appear that LPS molecules on B. japonicum effect the properties, which precipitate more distinctly metal-rich mineral phase.
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